Colorimeter Sensor   Home / SmartQ Sensors  

A simple, effective, recording colorimeter capable of monitoring changes in transmission or absorbance over time.

Ideal for teaching the characteristics of enzyme kinetics and chemical rates of reaction.

Uses a white LED, standard cuvettes and coloured slides (4 supplied) as filters. Use the meters function of Easysense it becomes a standard reading display colorimeter.

Milk and protein graph
Teaching applications:
  • Reaction kinetics
  • Reaction kinetics of enzyme systems
  • Rate of reaction with Thiosulphate and acid catalyst
 

Beer lamberts law graph
Extension and advanced ideas:
  • Beer Lamberts law (light absorbance vs. concentration)
  • Population growth of micro-organisms
  • Estimation of unknowns e.g. glucose concentration, manganese in paper clips

More Information

  • Colour filters are supplied as 35 mm slides, simple to add more filter colours.
  • Red (630nm), green (560nm), amber(600nm) and blue (470nm) filters supplied.
  • Uses standard 4 ml capacity square cuvettes.
  • 5 cuvettes and lids supplied (optical polystyrene).
  • LED light technology minimises drift.
  • Single calibration / zero control.

Contents/Details:

Transmittance: 0 to 110% (Resolution 0.1%) 
Absorbance: 0.0500 to 1.0500

Available Resources

Downloads Colorimeter Sensor Manual
Doc No.: DS042 | Issue: 5

Teaching Materials

Enzyme reaction (Protease) (Biology (11-14) ebook)
This is simple version of following an enzyme reaction. It could fit into work about digestion and digestive enzymes. Much more reliable than amylase and starch.

View Worksheet Biology (11-14) ebook
Beetroot membrane colorimeter (Biology (14-18) eBook)
Use a colorimeter to study the changes in a partially permeable membrane due to heat. Measure the intensity of the colour around the beetroot plugs. Could also be used to study membrane changes due to exposure to ethanol.

View Worksheet Biology (14-18) eBook
Protease digestion of Casein, simple enzyme study (Biology (14-18) eBook)
Us a colorimeter to follow the digestion of protein by a non specific protease over time. An introductory activity to get students used to the apparatus and method. Easier than amylase. Highly recommended.

View Worksheet Biology (14-18) eBook
Temperature and enzyme activity (protease) (Biology (14-18) eBook)
Milk powder suspension contains proteins that are poorly soluble giving the milk an opaque appearance. A non specific protease will digest the proteins and make the solution transparent. The progress of the reaction can be followed with a colorimeter. If the substrate is stored at different temperatures the effect of temperature on the reaction can be demonstrated.

View Worksheet Biology (14-18) eBook
pH and Enzyme activity (protease) (Biology (14-18) eBook)
Milk powder suspension is an opaque suspension of poorly soluble proteins. A non specific protease will digest the protein creating a transparent solution. A colorimeter can be used to follow the reaction. Adding drops of acid to the reaction allows study of the effect of pH on the rate,

View Worksheet Biology (14-18) eBook
Enzyme activity and substrate concentration (protease) (Biology (14-18) eBook)
In this investigation the effect of increasing the substrate concentration on the speed of the reaction is considered. Uses a non specific protease to digest milk proteins and change opacity of the reaction mix.

View Worksheet Biology (14-18) eBook
Inhibition of enzyme activity by cobalt (Biology (14-18) eBook)
Many of the transition metals act as inhibitors of enzyme systems; in part this explains the toxicity of these metals. The experiment uses cobalt as an inhibitor as it is a readily available metal salt. Ideal extension activity for enzyme work, once mastered, try other metal salts.

View Worksheet Biology (14-18) eBook
Model of Michaelis-Menten (Biology (14-18) eBook)
A demonstration of how the Michaelis constant could be found for an enzyme system. Good extension or project practical.

View Worksheet Biology (14-18) eBook
Enzyme action (Amylase) (Biology (14-18) eBook)
Use a colorimeter to follow the classic amylase digestion of starch.

View Worksheet Biology (14-18) eBook
Enzymes and temperature (amylase) (Biology (14-18) eBook)
Change the temperature of the substrate to study the effect of amylase on starch digestion. Uses a colorimeter to follow the change in colour of the starch-iodine complex.

View Worksheet Biology (14-18) eBook
Enzymes and pH (amylase) (Biology (14-18) eBook)
Use a colorimeter to follow the change of colour of the starch-iodine complex during the amylase digestion of starch. pH is the controlled variable.

View Worksheet Biology (14-18) eBook
Enzymes and substrate concentration (amylase) (Biology (14-18) eBook)
What is the effect of changing the substrate concentration on the amylase digestion of starch. Uses a colorimeter to follow the change in colour of the starch iodine complex as the digestion takes place

View Worksheet Biology (14-18) eBook
Estimation of glucose (colorimeter) (Biology (14-18) eBook)
An alternative method for measurement of glucose. Uses manganate solution and a colorimeter to record how long to decolourise. Application of Beer Lamberts law and creation of standards curves.

View Worksheet Biology (14-18) eBook
Glucose and Benedict's (Biology (14-18) eBook)
A more precise Benedict's test. Uses colorimeter to determine the depth of colour from the test. Application of Beer Lamberts law and use of calibration curves.

View Worksheet Biology (14-18) eBook
Why Does Fruit Brown (Biology (14-18) eBook)
look at why fruit browns using a colorimeter to create some numerical data for comparison.

View Worksheet Biology (14-18) eBook
Thiosulphate rate of reaction investigation. (Chemistry (14-18) eBook)
This investigation is about rates of reaction, Uses the classic thiosulphate and acid reaction, but follows the rate with a data logging colorimeter

View Worksheet Chemistry (14-18) eBook
Ethanedioate autocatalysis (Chemistry (14-18) eBook)
Manganate(VII) ions in acid solution oxidise ethanedioate ions to carbon dioxide. As manganate(VII) ions are coloured deep purple, the reaction can be followed colorimetrically by monitoring the loss of the colour as the reaction progresses.

View Worksheet Chemistry (14-18) eBook
Beer's Law (Chemistry (14-18) eBook)
This experiment is effectively a demonstration of the principles of Beer’s law. The applications are very wide spread and include biomedical monitoring to quality assurance of beer.

View Worksheet Chemistry (14-18) eBook
Estimation of chlorine In water. (Chemistry (14-18) eBook)
Chlorine is added to pool water to prevent the spread of waterborne diseases. Chlorine levels need to measured to make the water safe from microorganisms and safe enough to swim in.

View Worksheet Chemistry (14-18) eBook
How much magnesium in a paper clip. (Chemistry (14-18) eBook)
Colorimetry is an analytical technique used to determine the concentrations of coloured substances in solution. It relies on the fact that a coloured substance absorbs light in direct proportion to concentration.

View Worksheet Chemistry (14-18) eBook
Stoichometry Of copper And EDTA. (Chemistry (14-18) eBook)
Stoichiometry is the study in chemistry of the ratio of the combining proportions of particles in a reaction. In this investigation copper is added to EDTA and the ratios of combination determined by colour density

View Worksheet Chemistry (14-18) eBook
Chemical equilibria: Calculationg Kc (Chemistry (14-18) eBook)
When Fe3+ ions and SCN- ions are combined they form the FeSCN2+ ion that is coloured blood red. The three ions form equilibrium with each other. The coloured product of the reaction can be measured indirectly by its absorbance in a Colorimeterand hence the Kc calculated

View Worksheet Chemistry (14-18) eBook
Estimating glucose in a solution (Chemistry (14-18) eBook)
Uses the time for a permanganate solution to discolour to create a concentration graph for estimation of glucose.

View Worksheet Chemistry (14-18) eBook
Order No. : 3275

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